O‘SIMLIKKA XOS CRISPR VEKTORLARI VA ULARNING TEXNIK AFZALLIKLARI
Keywords:
CRISPR/Cas9, Csy4-gRNA, asosiy tahrirlash, multiplekslash, endonukleazalar.Abstract
CRISPR/Cas vositachiligida genomni tahrirlash o‘simlik genomini manipulyatsiya qilishning inqilobiy yondashuvidir. Biroq, ushbu texnologiyaning muvaffaqiyati aniq vektor va boshqa komponentlarni tanlashga bog‘liq. O‘simlikka xos CRISPR/Cas vektori odatda Cas dan iboratgen, maqsadga xos gRNK, yetakchi ketma-ketligi, tanlangan marker geni, aniq promouterlar va boshqa qismlar. Bir joyda CRISPR vektorlari haqida to‘liq ma’lumot yo‘qligi sababli har bir tadqiqot uchun o‘ziga xos vektorni tanlash har doim qiyin bo‘lgan. Bu erda biz vektor tanlashda va kerakli o‘simlik genomini samarali tahrirlashda juda foydali bo‘lgan turli xil muhim elementlarning har bir texnik jihatlarini muhokama qildik.
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