CRISPR-CAS9 TIZIMI YORDAMIDA GENLARNI TAHRIRLASH VA HOSILNI YAXSHILASH
Keywords:
CRISPR/Cas tizimi, genomni tahrirlash, ovqatlanishni yaxshilash, kasalliklarga chidamlilik, metabolik muhandislik, gen ekspressiyasini tartibga solish, CRISPR ribonukleoproteinlari.Abstract
Genomni tahrirlash texnologiyalaridagi yutuqlar funktsional genomika va hosilni yaxshilash sohalarida inqilob qildi. CRISPR/Cas9 (klasterli muntazam intervalgacha qisqa palindromik takrorlash) -Cas9 genetik muhandislik uchun ko‘p maqsadli texnologiya bo‘lib, u hidoyatRNK (gRNK) ning ma’lum bir ketma-ketlik va Cas9 endonukleaza faolligiga to‘ldirilishiga asoslanadi. Bu qishloq xo‘jaligining tadqiqot sohasini kengaytirdi, zararli xususiyatlarni yo‘q qilish yoki muhim belgilarni qo‘shish bilan yangi o‘simlik navlarini yaratish uchun yangi imkoniyatlar yaratdi. Ushbu RNK tomonidan boshqariladigan genomni tahrirlash texnologiyasi o‘simlik biologiyasining alohida sohalarida innovatsion yangilik bo‘lib chiqdi. CRISPR texnologiyasi doimiy ravishda rivojlanib bormoqda, jumladan nokautlar yaratish kabi turli genetik manipulyatsiyalar uchun variantlar; aniq o‘zgartirishlar kiritish, multipleks genom muhandisligi va maqsadli genlarni faollashtirish va repressiya qilish. Ko‘rib chiqish CRISPR merosi davomidagi taraqqiyotni ta’kidlaydi. Biz CRISPR/Cas9 vositalarining koʻp funksiyalari, imkoniyatlari va maxsus ilovalari bilan tez evolyutsiyasini oʻrganib chiqdik. Turli xil ishlar orasida o‘simliklarning oziqlanishini yaxshilash, o‘simliklarning kasalliklarga chidamliligini oshirish va qurg‘oqchilikka chidamli o‘simliklarni etishtirish ko‘rib chiqiladi. Sharh shuningdek, Cas9-gRNK komplekslarini o‘simlik hujayralariga etkazib berishning an’anaviy usullari haqida ba’zi ma’lumotlarni o‘z ichiga oladi va plazmidga asoslangan CRISPR tizimida mavjud bo‘lgan turli cheklovlarga yechim sifatida paydo bo‘lgan CRISPR ribonukleoproteinlarining (RNPs) paydo bo‘lishini o‘z ichiga oladi. Biz CRISPR/Cas9 vositalarining koʻp funksiyalari, imkoniyatlari va maxsus ilovalari bilan tez evolyutsiyasini oʻrganib chiqdik. Turli xil ishlar orasida o‘simliklarning oziqlanishini yaxshilash, o‘simliklarning kasalliklarga chidamliligini oshirish va qurg‘oqchilikka chidamli o‘simliklarni etishtirish ko‘rib chiqiladi. Sharh shuningdek, Cas9-gRNK komplekslarini o‘simlik hujayralariga etkazib berishning an’anaviy usullari haqida ba’zi ma’lumotlarni o‘z ichiga oladi va plazmidga asoslangan CRISPR tizimida mavjud bo‘lgan turli cheklovlarga yechim sifatida paydo bo‘lgan CRISPR ribonukleoproteinlarining (RNPs) paydo bo‘lishini o‘z ichiga oladi. Biz CRISPR/Cas9 vositalarining koʻp funksiyalari, imkoniyatlari va maxsus ilovalari bilan tez evolyutsiyasini oʻrganib chiqdik. Turli xil ishlar orasida o‘simliklarning oziqlanishini yaxshilash, o‘simliklarning kasalliklarga chidamliligini oshirish va qurg‘oqchilikka chidamli o‘simliklarni etishtirish ko‘rib chiqiladi. Sharh shuningdek, Cas9-gRNK komplekslarini o‘simlik hujayralariga etkazib berishning an’anaviy usullari haqida ba’zi ma’lumotlarni o‘z ichiga oladi va plazmidga asoslangan CRISPR tizimida mavjud bo‘lgan turli cheklovlarga yechim sifatida paydo bo‘lgan CRISPR ribonukleoproteinlarining (RNPs) paydo bo‘lishini o‘z ichiga oladi.
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